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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
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0
Conjugate
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验证数据展示

  • WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

    WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

    IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

  • WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

    WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82210-1-RR

    Affinity and Kinetic Characterization of 82210-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82210-1-RR against Human SIRT3 were performed. The affinity constant is 0.261 nM.

SIRT3 Recombinant antibody, PBS Only

SIRT3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82210-1-PBS

产品说明书

CloneNo. 2P6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, Indirect ELISA

别名

EC:2.3.1.286, hSIRT3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82210-1-PBS targets SIRT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SIRT3 fusion protein Ag26280 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
别名 EC:2.3.1.286, hSIRT3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3
计算分子量 44 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC001042
基因名称 SIRT3
Gene ID (NCBI) 23410
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NTG7
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.