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SIRT3 Recombinant antibody, PBS Only

SIRT3 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2P6

Cat no : 82210-1-PBS

Print datasheet

Synonyms

SIR2L3, SIR2 like protein 3, Regulatory protein SIR2 homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3

验证数据展示

  • WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

    WB analysis using 82210-1-RR (same clone as 82210-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82210-1-RR (SIRT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

    IHC staining of human prostate cancer using 82210-1-RR (same clone as 82210-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 82210-1-RR (SIRT3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82210-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82210-1-RR

    Affinity and Kinetic Characterization of 82210-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82210-1-RR against Human SIRT3 were performed. The affinity constant is 0.261 nM.

产品信息

82210-1-PBS targets SIRT3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SIRT3 fusion protein Ag26280 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
Synonyms SIR2L3, SIR2 like protein 3, Regulatory protein SIR2 homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3
Calculated Molecular Weight 44 kDa
Observed Molecular Weight28-30 kDa
GenBank Accession NumberBC001042
Gene Symbol SIRT3
Gene ID (NCBI) 23410
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NTG7
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.