验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, rat heart tissue, HepG2 cells, HT-29 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82210-1-RR targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Tested Reactivity | human, mouse, rat, pig |
| Immunogen | SIRT3 fusion protein Ag26280 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae) |
| Synonyms | SIR2L3, SIR2 like protein 3, Regulatory protein SIR2 homolog 3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, hSIRT3 |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 28-30 kDa |
| GenBank Accession Number | BC001042 |
| Gene Symbol | SIRT3 |
| Gene ID (NCBI) | 23410 |
| RRID | AB_3086470 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NTG7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIRT3 antibody 82210-1-RR | Download protocol |
| IHC protocol for SIRT3 antibody 82210-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
