SIRT3 Recombinant antibody

SIRT3 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 82210-1-RR

产品说明书

CloneNo. 2P6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, ELISA

EC:2.3.1.286, hSIRT3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, mouse heart tissue, rat heart tissue, HepG2 cells, HT-29 cells, mouse liver tissue, pig liver tissue, rat liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82210-1-RR targets SIRT3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 SIRT3 fusion protein Ag26280 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae)
别名 EC:2.3.1.286, hSIRT3, NAD-dependent protein deacetylase sirtuin-3, mitochondrial, Regulatory protein SIR2 homolog 3, SIR2 like protein 3
计算分子量 44 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC001042
基因名称 SIRT3
Gene ID (NCBI) 23410
RRIDAB_3086470
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NTG7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Sirtuins are NAD+-dependent histone/protein deacetylases (HDAC) and SIRT3 is the only sirtuin whose increased expression has been shown to correlate with an extended life span in humans. It is localized in the mitochondrial matrix, where it regulates the acetylation levels of metabolic enzymes, including acetyl coenzyme A synthetase 2. SIRT3 is stress-responsive and its increased expression protects myocytes from genotoxic and oxidative stress-mediated cell death. full-length hSIRT3 (44 kDa) is an inert protein and that it is activated inside the mitochondria following deletion of 142 amino acids of the N-terminal segment. This processed form of SIRT3 is approximately 28 kDa and possesses deacetylase activity. The full SIRT3 is a 44 kDa protein and is processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product.

实验方案

Product Specific Protocols
WB protocol for SIRT3 antibody 82210-1-RRDownload protocol
IHC protocol for SIRT3 antibody 82210-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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