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SIRT5 Recombinant antibody, PBS Only

SIRT5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

7L20

Cat no : 82246-1-PBS

Print datasheet

Synonyms

FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5

验证数据展示

  • WB analysis using 82246-1-RR (same clone as 82246-1-PBS)

    WB analysis using 82246-1-RR (same clone as 82246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82246-1-RR (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82246-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82246-1-RR (same clone as 82246-1-PBS)

    IF Staining of HeLa using 82246-1-RR (same clone as 82246-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIRT5 antibody (82246-1-RR, Clone: 7L20 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004). This data was developed using the same antibody clone with 82246-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82246-1-PBS

    Affinity and Kinetic Characterization of 82246-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82246-1-RR against Human SIRT5 were performed. The affinity constant is 1.64 nM.

产品信息

82246-1-PBS targets SIRT5 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen SIRT5 fusion protein Ag7195 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
Synonyms FLJ36950, SIR2 like protein 5, SIR2L5, SIRT5
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 32-33 kDa
GenBank Accession NumberBC000126
Gene Symbol SIRT5
Gene ID (NCBI) 23408
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NXA8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.