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验证数据展示

  • WB analysis using 82246-1-RR

    WB analysis using 82246-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82246-1-RR (SIRT5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 82246-1-RR

    IF Staining of HeLa using 82246-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SIRT5 antibody (82246-1-RR, Clone: 7L20 ) at dilution of 1:1200 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • Affinity and Kinetic Characterization of 82246-1-RR

    Affinity and Kinetic Characterization of 82246-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82246-1-RR against Human SIRT5 were performed. The affinity constant is 1.64 nM.

SIRT5 Recombinant antibody

SIRT5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 82246-1-RR

产品说明书

CloneNo. 7L20

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

7L20, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HepG2 cells, Jurkat cells, HCT 116 cells, RAW 264.7 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:600-1:2400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82246-1-RR targets SIRT5 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 SIRT5 fusion protein Ag7195 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 sirtuin (silent mating type information regulation 2 homolog) 5 (S. cerevisiae)
别名 7L20, EC:2.3.1.-, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2L5
计算分子量 34 kDa
观测分子量 32-33 kDa
GenBank蛋白编号BC000126
基因名称 SIRT5
Gene ID (NCBI) 23408
RRIDAB_3086471
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NXA8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRT5, a member fo sirtuin family that is a mitochondrial matrix NAD(+)-dependent deacetylase and mono-ADP-ribosyltransferases. Sirtuins play important role in various cellular processed, such as aging, gene silencing and metabolism. SIRT5 mainly locates in cytoplasm. It is an efficient protein lysine desuccinylase and demalonylase. During prolonged fasting, it can activate CPS1, the committed and regulated enzyme of the urea cycle, and this contributes to the regulation of blood ammonia levels.

实验方案

Product Specific Protocols
WB protocol for SIRT5 antibody 82246-1-RRDownload protocol
IF protocol for SIRT5 antibody 82246-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols