SIX1 Monoclonal antibody
SIX1 Monoclonal Antibody for WB, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, pig, rabbit, rat
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2D7D1
验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IF/ICC detected in | A431 cells |
Planning an IHC experiment? We recommend our IHCeasy SIX1 Ready-To-Use IHC Kit. SIX1 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67741-1-Ig targets SIX1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Tested Reactivity | Human, mouse, pig, rabbit, rat |
| Immunogen | SIX1 fusion protein Ag28570 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SIX homeobox 1 |
| Synonyms | BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39 |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 30-35 kDa |
| GenBank Accession Number | BC008874 |
| Gene Symbol | SIX1 |
| Gene ID (NCBI) | 6495 |
| RRID | AB_2918510 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q15475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SIX1 antibody 67741-1-Ig | Download protocol |
| IF protocol for SIX1 antibody 67741-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
