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验证数据展示

  • WB analysis using 67741-1-Ig

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67741-1-Ig

    WB analysis using 67741-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67741-1-Ig (SIX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 67741-1-Ig

    IF Staining of A431 using 67741-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SIX1 antibody (67741-1-Ig, Clone: 2D7D1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

SIX1 Monoclonal antibody

SIX1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67741-1-Ig

产品说明书

CloneNo. 2D7D1

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig, rabbit, rat

应用

WB, IF/ICC, ELISA

别名

BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, HeLa cells, rat skeletal muscle tissue, mouse skeletal muscle tissue
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy SIX1 Ready-To-Use IHC Kit. SIX1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67741-1-Ig targets SIX1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, pig, rabbit, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, pig, rabbit, rat
免疫原 SIX1 fusion protein Ag28570 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SIX homeobox 1
别名 BOS3, DFNA23, Homeobox protein SIX1, Sine oculis homeobox homolog 1, SIX homeobox 1, SIX1, TIP39
计算分子量 32 kDa
观测分子量30-35 kDa
GenBank蛋白编号BC008874
基因名称 SIX1
Gene ID (NCBI) 6495
RRIDAB_2918510
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15475
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIX1 belongs to SIX family that contains a divergent DNA-binding homeodomain and an upstream SIX domain, which may participate both in determining DNA-binding specificity and in mediating protein-protein interactions. It may be involved in limb tendon and ligament development, and specifically expressed in skeletal muscle. SIX1 plays a essential role in the development of numerous organs and shows littlt to no expression in most non-neoplastic adult tissue, yet is overexpression in a number of meoplasms where it increases cell proliferation and survival. The calcualted molecular weight of SIX1 is 32kDa, but modified SIX1 is about 35 kDa.

实验方案

Product Specific Protocols
WB protocol for SIX1 antibody 67741-1-IgDownload protocol
IF protocol for SIX1 antibody 67741-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols