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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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Conjugate
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验证数据展示

  • FC experiment of C2C12 using CL594-66347

    FC experiment of C2C12 using CL594-66347

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated SIX2 Monoclonal antibody

SIX2 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66347

产品说明书

CloneNo. 1F9G11

宿主/亚型

Mouse / IgG2b

种属反应性

human, rat, Canine, mouse, pig

应用

FC (Intra)

别名

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inC2C12 cells
Positive FC detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 66347-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66347 targets SIX2 in FC (Intra) applications and shows reactivity with human, rat, Canine, mouse, pig samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, rat, Canine, mouse, pig
免疫原 SIX2 fusion protein Ag2124 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 SIX homeobox 2
别名 Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2
计算分子量 291 aa, 32 kDa
观测分子量 37 kDa
GenBank蛋白编号BC024033
基因名称 SIX2
Gene ID (NCBI) 10736
RRIDAB_3084706
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9NPC8
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

实验方案

Product Specific Protocols
FC protocol for CL594 SIX2 antibody CL594-66347Download protocol
Standard Protocols
Click here to view our Standard Protocols