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验证数据展示

  • FC experiment of C2C12 using CL594-66347

    FC experiment of C2C12 using CL594-66347

    1X10^6 C2C12 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SIX2 (CL594-66347, Clone:1F9G11) (red), or 0.8 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

CoraLite®594-conjugated SIX2 Monoclonal antibody

SIX2 Monoclonal Antibody for FC (Intra)
Cat No. CL594-66347
CloneNo. 1F9G11

Host / Isotype

Mouse / IgG2b

Reactivity

human, rat, Canine, mouse, pig

Applications

FC (Intra)

Synonyms

Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inC2C12 cells
Positive FC detected inC2C12 cells
For other applications, we recommend the unconjugated version of this antibody, 66347-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66347 targets SIX2 in FC (Intra) applications and shows reactivity with human, rat, Canine, mouse, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, rat, Canine, mouse, pig
Immunogen SIX2 fusion protein Ag2124 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name SIX homeobox 2
Synonyms Homeobox protein SIX2, Sine oculis homeobox homolog 2, SIX homeobox 2, SIX2
Calculated Molecular Weight 291 aa, 32 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC024033
Gene Symbol SIX2
Gene ID (NCBI) 10736
RRIDAB_3084706
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NPC8
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The SIX proteins (sine oculis) are a family of homeodomain transcription factors that share a conserved DNA binding domain. Six members (Six1-Six6) of the Six gene family have been identified in mice and humans. SIX2, containing one homeobox DNA-binding domain, is highly expressed in fetal tissues but expression is limited in adult tissues. SIX2 may be involved in limb tendon and ligament development[PMID:21420949]. It has been previously shown that SIX2 is expressed in developing mesenchymal tissue including head and urogenital system at the time of overt midfacial and renal differentiation[PMID: 22282599].

实验方案

Product Specific Protocols
FC protocol for CL594 SIX2 antibody CL594-66347Download protocol
Standard Protocols
Click here to view our Standard Protocols