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SIX5 Polyclonal antibody

SIX5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22938-1-AP

Print datasheet

Synonyms

BOR2, DMAHP, Homeobox protein SIX5, Sine oculis homeobox homolog 5, SIX homeobox 5, SIX5

验证数据展示

  • WB analysis of Y79 using 22938-1-AP

    WB analysis of Y79 using 22938-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 22938-1-AP (SIX5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of Y79 using 22938-1-AP

    IP experiment of Y79 using 22938-1-AP

    IP result of anti-SIX5 (IP:22938-1-AP, 4ug; Detection:22938-1-AP 1:300) with Y79 cells lysate 2000ug.

  • IHC staining of human kidney using 22938-1-AP

    IHC staining of human kidney using 22938-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22938-1-AP (SIX5 Antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with SIX5 antibody included
  • WB analysis of HeLa using 22938-1-AP

    WB analysis of HeLa using 22938-1-AP

    WB result of SIX5 antibody (22938-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SIX5 transfected HeLa cells.

经过测试的应用

Positive WB detected inY79 cells, HeLa cells
Positive IP detected inY79 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy SIX5 Ready-To-Use IHC Kit. SIX5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

22938-1-AP targets SIX5 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen SIX5 fusion protein Ag18993 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SIX homeobox 5
Synonyms BOR2, DMAHP, Homeobox protein SIX5, Sine oculis homeobox homolog 5, SIX homeobox 5, SIX5
Calculated Molecular Weight 739 aa, 75 kDa
Observed Molecular Weight 100-105 kDa
GenBank Accession NumberBC156983
Gene Symbol SIX5
Gene ID (NCBI) 147912
RRIDAB_2879186
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N196
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIX5 antibody 22938-1-APDownload protocol
IHC protocol for SIX5 antibody 22938-1-APDownload protocol
IP protocol for SIX5 antibody 22938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Immunity

An atlas of cells in the human tonsil

Authors - Ramon Massoni-Badosa
View Article
mouseWB

iScience

RBFOX2 regulated EYA3 isoforms partner with SIX4 or ZBTB1 to control transcription during myogenesis

Authors - Hannah J Wiedner
View Article