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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis of RAW 264.7 using 66367-1-Ig (same clone as 66367-1-PBS)

    WB analysis of RAW 264.7 using 66367-1-Ig (same clone as 66367-1-PBS)

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66367-1-Ig (same clone as 66367-1-PBS)

    IHC staining of human colon cancer using 66367-1-Ig (same clone as 66367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66367-1-Ig (same clone as 66367-1-PBS)

    IHC staining of human colon cancer using 66367-1-Ig (same clone as 66367-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 66367-1-Ig (same clone as 66367-1-PBS)

    IF Staining of NIH/3T3 using 66367-1-Ig (same clone as 66367-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SKAP2 antibody (66367-1-Ig, Clone: 2G3G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 66367-1-PBS in a different storage buffer formulation.

SKAP2 Monoclonal antibody, PBS Only

SKAP2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66367-1-PBS

产品说明书

CloneNo. 2G3G9

宿主/亚型

Mouse / IgG1

种属反应性

human, pig, rat, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66367-1-PBS targets SKAP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rat, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig, rat, mouse
免疫原 SKAP2 fusion protein Ag3615 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 src kinase associated phosphoprotein 2
别名 PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R
计算分子量 41 kDa
观测分子量 55 kDa
GenBank蛋白编号BC036044
基因名称 SKAP2
Gene ID (NCBI) 8935
RRIDAB_2881747
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75563
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.