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SKAP2 Monoclonal antibody

SKAP2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2G3G9

Cat no : 66367-1-Ig

Print datasheet

Synonyms

PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R

验证数据展示

  • WB analysis of RAW 264.7 using 66367-1-Ig

    WB analysis of RAW 264.7 using 66367-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66367-1-Ig (SKAP2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 66367-1-Ig

    IHC staining of human colon cancer using 66367-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 66367-1-Ig

    IHC staining of human colon cancer using 66367-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66367-1-Ig (SKAP2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 66367-1-Ig

    IF Staining of NIH/3T3 using 66367-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using SKAP2 antibody (66367-1-Ig, Clone: 2G3G9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inRAW 264.7 cells, human spleen tissue, pig liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66367-1-Ig targets SKAP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rat, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, pig, rat, mouse
Immunogen SKAP2 fusion protein Ag3615 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name src kinase associated phosphoprotein 2
Synonyms PRAP, Pyk2/RAFTK associated protein, RA70, SAPS, SCAP2, SKAP 55HOM, SKAP HOM, SKAP2, SKAP55 homolog, SKAP55R
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC036044
Gene Symbol SKAP2
Gene ID (NCBI) 8935
RRIDAB_2881747
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75563
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.

实验方案

Product Specific Protocols
WB protocol for SKAP2 antibody 66367-1-IgDownload protocol
IHC protocol for SKAP2 antibody 66367-1-IgDownload protocol
IF protocol for SKAP2 antibody 66367-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols