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验证数据展示

  • FC experiment of NIH/3T3 using CL488-66367

    FC experiment of NIH/3T3 using CL488-66367

    1X10^6 NIH/3T3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SKAP2 (CL488-66367, Clone:2G3G9) (red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of NIH/3T3 using CL488-66367

    IF Staining of NIH/3T3 using CL488-66367

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CoraLite® Plus 488 SKAP2 antibody (CL488-66367, Clone: 2G3G9 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated SKAP2 Monoclonal antibody

SKAP2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66367

CloneNo.

2G3G9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Synonyms

Retinoic acid-induced protein 70, RA70, Pyk2/RAFTK-associated protein, Pyk2/RAFTK associated protein, PRAP

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inNIH/3T3 cells
Positive FC (Intra) detected inNIH/3T3 cells
For other applications, we recommend the unconjugated version of this antibody, 66367-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66367 targets SKAP2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SKAP2 fusion protein Ag3615 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name src kinase associated phosphoprotein 2
Synonyms Retinoic acid-induced protein 70, RA70, Pyk2/RAFTK-associated protein, Pyk2/RAFTK associated protein, PRAP
Calculated Molecular Weight 41 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC036044
Gene Symbol SKAP2
Gene ID (NCBI) 8935
RRIDAB_3084239
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75563
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SKAP2 (src kinase associated phosphoprotein 2), also named PRAP, contains PH domain and SH3 domain. It is localized in cytoplasm and was expressed in ubiquitously in various tissues including lung, skeletal muscle, and spleen, but not detectable in thymus and T cell lymphoma cells. SKAP2 may negatively regulate alpha-synuclein phosphorylation following cell stress. SKAP2 may be involved in B-cell and macrophage adhesion processes by coupling the B-cell receptor (BCR) to integrin activation.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SKAP2 antibody CL488-66367Download protocol
FC protocol for CL Plus 488 SKAP2 antibody CL488-66367Download protocol
Standard Protocols
Click here to view our Standard Protocols