验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SKP2 Ready-To-Use IHC Kit. SKP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 15010-1-AP
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-15010 targets SKP2 in IF/ICC applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | SKP2 fusion protein Ag6826 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | S-phase kinase-associated protein 2 (p45) |
| 别名 | F box protein Skp2, F box/LRR repeat protein 1, FBL1, FBXL1, FLB1, p45skp2, SKP2 |
| 计算分子量 | 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC001441 |
| 基因名称 | SKP2 |
| Gene ID (NCBI) | 6502 |
| RRID | AB_3083967 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13309 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SKP2(S-phase kinase-associated protein 2) is also named as FBXL1 and belongs to the F-box family. It regulates cellular proliferation by targeting several cell cycle-regulated proteins for ubiquitination and degradation, including cyclin-dependent kinase inhibitor CDKN1B(PMID:22200179). The acetylation of SKP2 in the nuclear localization signal (NLS) can promote its cytoplasmic retention, and cytoplasmic SKP2 enhances cellular migration through ubiquitination and destruction of CDH. This protein has 3 isoforms produced by alternative splicing (PMID:26497688, PMID:16902410).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SKP2 antibody CL488-15010 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |