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Anti-Human SLAM/CD150 Rabbit Recombinant Antibody

SLAM/CD150 Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

241800B12

Cat no : 98220-1-RR

Print datasheet

Synonyms

SLAMF1, SLAM family member 1, SLAM, IPO-3, IPO 3

验证数据展示

  • FC experiment of human PBMCs using 98220-1-RR

    FC experiment of human PBMCs using 98220-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human SLAM/CD150 Rabbit Recombinant Antibody (98220-1-RR, Clone: 241800B12) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • FC experiment of human PBMCs using 98220-1-RR

    FC experiment of human PBMCs using 98220-1-RR

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human SLAM/CD150 Rabbit Recombinant Antibody (98220-1-RR, Clone: 241800B12) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

  • Affinity and Kinetic Characterization of 98220-1-RR

    Affinity and Kinetic Characterization of 98220-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98220-1-RR against Human SLAM/CD150 were performed. The affinity constant is 3.16 nM.

经过测试的应用

Positive FC detected inhuman PBMCs

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98220-1-RR targets SLAM/CD150 in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signaling lymphocytic activation molecule family member 1
Synonyms SLAMF1, SLAM family member 1, SLAM, IPO-3, IPO 3
Calculated Molecular Weight37kDa
GenBank Accession NumberNM_003037.5
Gene Symbol CD150
Gene ID (NCBI) 6504
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13291-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

实验方案

Product Specific Protocols
FC protocol for SLAM/CD150 antibody 98220-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols