验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human brain tissue, rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse cerebellum tissue |
Planning an IHC experiment? We recommend our IHCeasy SLC12A5 Ready-To-Use IHC Kit. SLC12A5 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
19565-1-AP targets KCC2/SLC12A5-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 12 (potassium-chloride transporter), member 5 |
| 别名 | hKCC2, K Cl cotransporter 2, KCC2, KCC2/SLC12A5-Specific, KIAA1176, Neuronal K Cl cotransporter, SLC12A5 |
| 计算分子量 | 126 kDa |
| 观测分子量 | 130-150 kDa |
| GenBank蛋白编号 | NM_020708 |
| 基因名称 | KCC2 |
| Gene ID (NCBI) | 57468 |
| RRID | AB_10638485 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9H2X9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
| IHC protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
| IF protocol for KCC2/SLC12A5-Specific antibody 19565-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neurobiol Dis HIV and opiates dysregulate K+- Cl- cotransporter 2 (KCC2) to cause GABAergic dysfunction in primary human neurons and Tat-transgenic mice. | ||
iScience Time-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway. | ||
ASN Neuro Restoration of KCC2 Membrane Localization in Striatal Dopamine D2 Receptor-Expressing Medium Spiny Neurons Rescues Locomotor Deficits in HIV Tat-Transgenic Mice. | ||
Brain Behav Involvement of the BDNF-TrkB-KCC2 pathway in neuropathic pain after brachial plexus avulsion. | ||
Front Mol Neurosci Partially brain effects of injection of human umbilical cord mesenchymal stem cells at injury sites in a mouse model of thoracic spinal cord contusion | ||
Ecotoxicol Environ Saf 1,2-Dichloroethane causes anxiety and cognitive dysfunction in mice by disturbing GABA metabolism and inhibiting the cAMP-PKA-CREB signaling pathway |