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验证数据展示

  • IF Staining of mouse cerebellum using CL488-19565

    IF Staining of mouse cerebellum using CL488-19565

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 KCC2/SLC12A5-Specific antibody (CL488-19565) at dilution of 1:200.

CoraLite® Plus 488-conjugated KCC2/SLC12A5-Specific Polyclonal antibody

KCC2/SLC12A5-Specific Polyclonal Antibody for IF-P
Cat No. CL488-19565

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

hKCC2, K Cl cotransporter 2, KCC2, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SLC12A5 Ready-To-Use IHC Kit. SLC12A5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19565-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19565 targets KCC2/SLC12A5-Specific in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 12 (potassium-chloride transporter), member 5
别名 hKCC2, K Cl cotransporter 2, KCC2, KIAA1176, Neuronal K Cl cotransporter, SLC12A5
计算分子量 126 kDa
观测分子量 130-150 kDa
GenBank蛋白编号NM_020708
基因名称 KCC2
Gene ID (NCBI) 57468
RRIDAB_3084025
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2X9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KCC2/SLC12A5-Specific antibody CL488-19565Download protocol
Standard Protocols
Click here to view our Standard Protocols