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验证数据展示

  • IF Staining of mouse cerebellum using CL488-19565

    IF Staining of mouse cerebellum using CL488-19565

    Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using CoraLite® Plus 488 KCC2/SLC12A5-Specific antibody (CL488-19565) at dilution of 1:200.

CoraLite® Plus 488-conjugated KCC2/SLC12A5-Specific Polyclonal antibody

KCC2/SLC12A5-Specific Polyclonal Antibody for IF-P
Cat No. CL488-19565

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

hKCC2, K Cl cotransporter 2, KCC2, KIAA1176, Neuronal K Cl cotransporter, SLC12A5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse cerebellum tissue
Planning an IHC experiment? We recommend our IHCeasy SLC12A5 Ready-To-Use IHC Kit. SLC12A5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 19565-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19565 targets KCC2/SLC12A5-Specific in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 12 (potassium-chloride transporter), member 5
Synonyms hKCC2, K Cl cotransporter 2, KCC2, KIAA1176, Neuronal K Cl cotransporter, SLC12A5
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 130-150 kDa
GenBank Accession NumberNM_020708
Gene Symbol KCC2
Gene ID (NCBI) 57468
RRIDAB_3084025
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2X9
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KCC2, encoded by SLC12A5, is a neuron-specific K+-Cl- cotransporter that is essential for Cl- homeostasis and inhibitory synaptic transmission in brain. KCC2 is expressed at high levels in neurons throughout the nervous system. KCC2 deletion caused animal death. Alterations of KCC2 have been linked to neuropathic pain, temporal lobe epilepsy, and other diseases. This antibody can detect 130-150 kDa monomer and ~250 kDa dimer of KKC2 in brain lysate. (PMID: 23401525)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 KCC2/SLC12A5-Specific antibody CL488-19565Download protocol
Standard Protocols
Click here to view our Standard Protocols