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  • Featured Product
  • KD/KO Validated

MCT4 Polyclonal antibody

MCT4 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

Conjugate

Unconjugated

Cat no : 22787-1-AP

Print datasheet

Synonyms

SLC16A3, MCT-4, MCT3, MCT 4

验证数据展示

  • WB analysis of HeLa using 22787-1-AP

    WB analysis of HeLa using 22787-1-AP

    WB result of MCT4 antibody (22787-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCT4 transfected HeLa cells.

  • WB analysis using 22787-1-AP

    WB analysis using 22787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 22787-1-AP

    IF Staining of HepG2 using 22787-1-AP

    Immunofluorescent analysis of HepG2 cells using 22787-1-AP (MCT4 antibody) at dilution of 1:50 and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human prostate cancer using 22787-1-AP

    IHC staining of human prostate cancer using 22787-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCT4 antibody included
  • IHC staining of human prostate cancer using 22787-1-AP

    IHC staining of human prostate cancer using 22787-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22787-1-AP (MCT4 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCT4 antibody included
  • FC experiment of HepG2 using 22787-1-AP

    FC experiment of HepG2 using 22787-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.8 ug Anti-Human MCT4 (22787-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 22787-1-AP

    WB analysis using 22787-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22787-1-AP (MCT4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 22787-1-AP

    IHC staining of human renal cell carcinoma using 22787-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCT4 antibody included
  • IHC staining of mouse skeletal muscle using 22787-1-AP

    IHC staining of mouse skeletal muscle using 22787-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCT4 antibody included
  • IHC staining of human breast cancer using 22787-1-AP

    IHC staining of human breast cancer using 22787-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22787-1-AP (MCT4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with MCT4 antibody included
  • IF Staining of HepG2 using 22787-1-AP

    IF Staining of HepG2 using 22787-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HepG2 using 22787-1-AP

    IF Staining of HepG2 using 22787-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of HepG2 using 22787-1-AP

    IF Staining of HepG2 using 22787-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using MCT4 antibody (22787-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

经过测试的应用

Positive WB detected inHeLa cells, rat skeletal muscle tissue, HepG2 cells, PC-3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue, human renal cell carcinoma tissue, mouse skeletal muscle tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCT4 Ready-To-Use IHC Kit. MCT4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22787-1-AP targets MCT4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat
Immunogen MCT4 fusion protein Ag18788 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
Synonyms SLC16A3, MCT-4, MCT3, MCT 4
Calculated Molecular Weight 465 aa, 49 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC112269
Gene Symbol MCT4
Gene ID (NCBI) 9123
RRIDAB_11182479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO15427
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

实验方案

Product Specific Protocols
WB protocol for MCT4 antibody 22787-1-APDownload protocol
IHC protocol for MCT4 antibody 22787-1-APDownload protocol
IF protocol for MCT4 antibody 22787-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Metab

Acetate enables metabolic fitness and cognitive performance during sleep disruption

Authors - Qinqin He
View Article
mouseWB

Nat Cancer

Targeting the bicarbonate transporter SLC4A4 overcomes immunosuppression and immunotherapy resistance in pancreatic cancer

Authors - Federica Cappellesso
View Article
humanWB

Nat Metab

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma

Authors - Zijian Yang
  • KD Validated
View Article

Adv Sci (Weinh)

SETDB1 Methylates MCT1 Promoting Tumor Progression by Enhancing the Lactate Shuttle

Authors - Xiaowei She
View Article
mouseIF

Nat Commun

Lactate sensing mechanisms in arterial chemoreceptor cells.

Authors - Hortensia Torres-Torrelo
View Article
mouseWB

Adv Healthc Mater

Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature.

Authors - Zhijie Jiang
View Article