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验证数据展示

  • IF Staining of HepG2 using CL488-22787

    IF Staining of HepG2 using CL488-22787

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 MCT4 antibody (CL488-22787) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-22787

    FC experiment of HepG2 using CL488-22787

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human MCT4 (CL488-22787) (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated MCT4 Polyclonal antibody

MCT4 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-22787

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

IF/ICC, FC (Intra)

别名

SLC16A3, MCT3, MCT 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy MCT4 Ready-To-Use IHC Kit. MCT4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 22787-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-22787 targets MCT4 in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, rat
免疫原 MCT4 fusion protein Ag18788 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 16, member 3 (monocarboxylic acid transporter 4)
别名 SLC16A3, MCT3, MCT 4
计算分子量 465 aa, 49 kDa
观测分子量 38-42 kDa
GenBank蛋白编号BC112269
基因名称 MCT4
Gene ID (NCBI) 9123
RRIDAB_3084070
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15427
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The monocarboxylate transporter 4 (MCT4, also known as SLC16A3) is involved in the transportation of metabolically important monocarboxylates such as lactate, pyruvate, acetate and ketone bodies. It is widely expressed, particularly strongly in glycolytic tissues such as white skeletal muscle fibres, astrocytes, white blood cells, chondrocytes and some mammalian cell lines. MCT4 is also linked to tumor biology because it mediates lactate transport across membranes resulting in antiapoptotic effects.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 MCT4 antibody CL488-22787Download protocol
FC protocol for CL Plus 488 MCT4 antibody CL488-22787Download protocol
Standard Protocols
Click here to view our Standard Protocols