• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

SLC1A5/ASCT2 Polyclonal antibody, PBS Only

SLC1A5/ASCT2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 20350-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 20350-1-AP (same clone as 20350-1-PBS)

    WB analysis using 20350-1-AP (same clone as 20350-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A5/ASCT2 antibody included
  • IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20350-1-AP (ASCT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A5/ASCT2 antibody included
  • IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using SLC1A5/ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of HEK-293 using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (20350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 20350-1-AP (same clone as 20350-1-PBS)

    FC experiment of HeLa using 20350-1-AP (same clone as 20350-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (20350-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SLC1A5/ASCT2 Ready-To-Use IHC Kit. SLC1A5/ASCT2 primary antibody included.

产品信息

20350-1-PBS targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse
Immunogen SLC1A5/ASCT2 fusion protein Ag14182 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 1 (neutral amino acid transporter), member 5
Synonyms
Calculated Molecular Weight 541 aa, 57 kDa
Observed Molecular Weight 55-70 kDa
GenBank Accession NumberBC000062
Gene Symbol SLC1A5/ASCT2
Gene ID (NCBI) 6510
RRIDAB_2878679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15758
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).