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验证数据展示

  • WB analysis using 20350-1-AP (same clone as 20350-1-PBS)

    WB analysis using 20350-1-AP (same clone as 20350-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20350-1-AP (SLC1A5/ASCT2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A5/ASCT2 antibody included
  • IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    IHC staining of human colon cancer using 20350-1-AP (same clone as 20350-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20350-1-AP (ASCT2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SLC1A5/ASCT2 antibody included
  • IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using SLC1A5/ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of mouse small intestine using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse small intestine tissue using ASCT2 antibody (20350-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), ZG16 antibody (67389-1-Ig, Clone: 1A7B9, red), CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7, Magenta). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • IF Staining of HEK-293 using 20350-1-AP (same clone as 20350-1-PBS)

    IF Staining of HEK-293 using 20350-1-AP (same clone as 20350-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SLC1A5/ASCT2 antibody (20350-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 20350-1-AP (same clone as 20350-1-PBS)

    FC experiment of HeLa using 20350-1-AP (same clone as 20350-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human SLC1A5/ASCT2 (20350-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 20350-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SLC1A5/ASCT2 Polyclonal antibody, PBS Only

SLC1A5/ASCT2 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA
Cat No. 20350-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SLC1A5/ASCT2 Ready-To-Use IHC Kit. SLC1A5/ASCT2 primary antibody included.

产品信息

20350-1-PBS targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 SLC1A5/ASCT2 fusion protein Ag14182 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 1 (neutral amino acid transporter), member 5
别名
计算分子量 541 aa, 57 kDa
观测分子量 55-70 kDa
GenBank蛋白编号BC000062
基因名称 SLC1A5/ASCT2
Gene ID (NCBI) 6510
RRIDAB_2878679
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15758
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).