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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5H7

Cat no : CL594-80008

Print datasheet

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

验证数据展示

  • IF Staining of mouse heart using CL594-80008

    IF Staining of mouse heart using CL594-80008

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

  • IF Staining of H9C2 using CL594-80008

    IF Staining of H9C2 using CL594-80008

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

  • FC experiment of C2C12 using CL594-80008

    FC experiment of C2C12 using CL594-80008

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inH9C2 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy smooth muscle actin Ready-To-Use IHC Kit. smooth muscle actin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 80008-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-80008 targets smooth muscle actin specific in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name actin, alpha 2, smooth muscle, aorta
Synonyms ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form
Calculated Molecular Weight 42 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberNM_001613
Gene Symbol Alpha smooth muscle actin
Gene ID (NCBI) 59
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP62736
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

实验方案

Product Specific Protocols
IF protocol for CL594 smooth muscle actin specific antibody CL594-80008Download protocol
FC protocol for CL594 smooth muscle actin specific antibody CL594-80008Download protocol
Standard Protocols
Click here to view our Standard Protocols