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验证数据展示

  • WB analysis using 67635-1-Ig

    WB analysis using 67635-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67635-1-Ig (SLC25A17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human liver cancer using 67635-1-Ig

    IHC staining of human liver cancer using 67635-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67635-1-Ig (SLC25A17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SLC25A17 Monoclonal antibody

SLC25A17 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67635-1-Ig

产品说明书

CloneNo. 2D1D2

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

WB, IHC, ELISA

别名

PMP34, SLC25A17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, U2OS cells, A549 cells, HeLa cells, HepG2 cells, K-562 cells, Jurkat cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67635-1-Ig targets SLC25A17 in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, Mouse
免疫原 SLC25A17 fusion protein Ag24456 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; peroxisomal membrane protein, 34kDa), member 17
别名 PMP34, SLC25A17
计算分子量 307 aa, 35 kDa
观测分子量 35 kDa
GenBank蛋白编号BC012998
基因名称 SLC25A17
Gene ID (NCBI) 10478
RRIDAB_2882836
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43808
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A17 (solute carrier family 25 member 17), is a kind of peroxisomal transporter for multiple cofactors such as CoA, FAD, FMN and AMP. It May catalyze the transport of free CoA, FAD and NAD+ from the cytosol into the peroxisomal matrix by a counter-exchange mechanism. SLC25A17 is the only member of the mitochondrial carrier family localized to peroxisomal. (PMID: 22185573, 32266253)

实验方案

Product Specific Protocols
WB protocol for SLC25A17 antibody 67635-1-IgDownload protocol
IHC protocol for SLC25A17 antibody 67635-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols