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验证数据展示

  • IF Staining of mouse brain using CL488-25402

    IF Staining of mouse brain using CL488-25402

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 SLC25A22 antibody (CL488-25402) at dilution of 1:200.

CoraLite® Plus 488-conjugated SLC25A22 Polyclonal antibody

SLC25A22 Polyclonal Antibody for IF-P
Cat No. CL488-25402

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF-P

别名

FLJ13044, GC 1, GC1, Glutamate/H(+) symporter 1, SLC25A22

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse brain tissue
For other applications, we recommend the unconjugated version of this antibody, 25402-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25402 targets SLC25A22 in IF-P applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 human, mouse, rat
免疫原 SLC25A22 fusion protein Ag19958 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier: glutamate), member 22
别名 FLJ13044, GC 1, GC1, Glutamate/H(+) symporter 1, SLC25A22
计算分子量 323 aa, 34 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC023545
基因名称 SLC25A22
Gene ID (NCBI) 79751
RRIDAB_3084090
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H936
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A22 encodes a mitochondrial glutamate/H+ symporter with highly expression in brain, especially in astrocytes, where it controls glutamate uptake. The mutations of SLC25A22 are associated with the early/infantile epileptic encephalopathies. It has been shown that SLC25A22 plays a key role in promoting proliferation and migration in cancer, such as human colorectal cancer (CRC) and Gallbladder cancer (GBC). And there is a result that the expression of SLC25A22 in GBC was significantly higher than that in adjacent tissues. 25402-1-AP antibody is specific to SLC25A22.(PMID: 30814911, 25033742, 24596948)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SLC25A22 antibody CL488-25402Download protocol
Standard Protocols
Click here to view our Standard Protocols