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验证数据展示

  • WB analysis using 82895-2-RR

    WB analysis using 82895-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82895-2-RR (SLC30A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of U2OS using 82895-2-RR

    FC experiment of U2OS using 82895-2-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SLC30A1 Recombinant antibody (82895-2-RR, Clone:230152E10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82895-2-RR

    Affinity and Kinetic Characterization of 82895-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82895-2-RR against Human SLC30A1 were performed. The affinity constant is 1.49 nM.

SLC30A1 Recombinant antibody

SLC30A1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82895-2-RR
CloneNo. 230152E10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

ZnT-1, ZNT1, Zinc transporter 1, 230152E10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inSW480 cells, HeLa cells, HepG2 cells
Positive FC (Intra) detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82895-2-RR targets SLC30A1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SLC30A1 fusion protein Ag18567 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 30 (zinc transporter), member 1
Synonyms ZnT-1, ZNT1, Zinc transporter 1, 230152E10
Calculated Molecular Weight 507 aa, 55 kDa
Observed Molecular Weight63~75 kDa
GenBank Accession NumberBC121015
Gene Symbol SLC30A1
Gene ID (NCBI) 7779
RRIDAB_3670624
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y6M5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Zinc transporter 1 (ZNT1), also known as SLC30A1, is the only zinc transporter predominantly located on the plasma membrane, where it plays a pivotal role exporting cytosolic zinc to the extracellular space. SLC30A1 expression is posttranslationally regulated by cellular zinc status and can be N-glycosylated on Asn299 in the extracellular loop between transmembrane domains (PMID: 15451416, 18936201). Western analysis detected a higher band at ~63 kDa than the expected molecular mass, which could be attributed to the properties of the cytosolic histidine-rich loop (PMID: 31471319).

实验方案

Product Specific Protocols
WB protocol for SLC30A1 antibody 82895-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols