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验证数据展示

  • FC experiment of HeLa using CL488-67993

    FC experiment of HeLa using CL488-67993

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SLC30A2 (CL488-67993, Clone:1D9E5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SLC30A2 Monoclonal antibody

SLC30A2 Monoclonal Antibody for FC (Intra)
Cat No. CL488-67993

产品说明书

CloneNo. 1D9E5

宿主/亚型

Mouse / IgG1

种属反应性

Human, Mouse

应用

FC (Intra)

别名

ZnT-2, ZNT2, ZnT 2, Proton-coupled zinc antiporter SLC30A2, PP12488

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67993-1-Ig

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67993 targets SLC30A2 in FC (Intra) applications and shows reactivity with Human, Mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 Human, Mouse
免疫原 SLC30A2 fusion protein Ag8232 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 30 (zinc transporter), member 2
别名 ZnT-2, ZNT2, ZnT 2, Proton-coupled zinc antiporter SLC30A2, PP12488
计算分子量 323 aa, 35 kDa
观测分子量41 kDa
GenBank蛋白编号BC006251
基因名称 SLC30A2
Gene ID (NCBI) 7780
RRIDAB_3084390
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRI3
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC30A2, also named as the Zn transporter 2 gene (ZnT2), belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family and SLC30A subfamily. It plays a role in the zinc ion transmembrane transport. Expression of SLC30A2 is restricted to secretory cells, such as acinar pancreatic cells, prostate epithelial cells, placental trophoblasts, Paneth cells, and mammary epithelial cells (MECs). SLC30A2 consists of six transmembrane domains with cytoplasmic N- and C-termini that contain numerous regulatory domains, and functions as a homo- or hetero- dimer to transport Zn into vesicles (PMID: 29476070 ). SLC30A2 has 2 isoforms with the molecular mass of 35 and 41 kDa.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SLC30A2 antibody CL488-67993Download protocol
Standard Protocols
Click here to view our Standard Protocols