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SLC43A1 Polyclonal antibody

SLC43A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10444-1-AP

Print datasheet

Synonyms

LAT3, PB39, POV1, R00504, SLC43A1

验证数据展示

  • WB analysis using 10444-1-AP

    WB analysis using 10444-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10444-1-AP (SLC43A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 10444-1-AP

    IHC staining of human kidney using 10444-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10444-1-AP (SLC43A1 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 10444-1-AP

    WB analysis using 10444-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10444-1-AP (SLC43A1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inPC-3 cells, Daudi cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

10444-1-AP targets SLC43A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SLC43A1 fusion protein Ag0305 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 43, member 1
Synonyms LAT3, PB39, POV1, R00504, SLC43A1
Calculated Molecular Weight 61 kDa
Observed Molecular Weight55-61 kDa
GenBank Accession NumberBC001639
Gene Symbol SLC43A1
Gene ID (NCBI) 8501
RRIDAB_2302076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75387
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC43A1, also named as LAT3, PB39 and POV1, belongs to the system L family of plasma membrane carrier proteins that transports large neutral amino acids. It plays a role in the development of human prostate cancer, from prostatic intraepithelial neoplasia to invasive prostate cancer.

实验方案

Product Specific Protocols
WB protocol for SLC43A1 antibody 10444-1-APDownload protocol
IHC protocol for SLC43A1 antibody 10444-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Arch Biochem Biophys

Enhancement of chemoresistance by claudin-1-mediated formation of amino acid barriers in human lung adenocarcinoma A549 cells

Authors - Riho Kimura
View Article