Band 3/AE 1 Polyclonal antibody

Band 3/AE 1 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28131-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA and More (2)

band 3, band 3/ AE1, SLC4A1, Band 3 anion transport protein, Anion exchanger 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, mouse liver tissue
Positive IHC detected inhuman spleen tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28131-1-AP targets Band 3/AE 1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Band 3/AE 1 fusion protein Ag27878 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
别名 band 3, band 3/ AE1, SLC4A1, Band 3 anion transport protein, Anion exchanger 1
计算分子量 911 aa, 102 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC101570
基因名称 band 3
Gene ID (NCBI) 6521
RRIDAB_2881069
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP02730
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

实验方案

Product Specific Protocols
WB protocol for Band 3/AE 1 antibody 28131-1-APDownload protocol
IHC protocol for Band 3/AE 1 antibody 28131-1-APDownload protocol
IF protocol for Band 3/AE 1 antibody 28131-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Cell

The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.

Authors - Eunus S Ali
humanIP

Int J Hematol

Band 3 ectopic expression in colorectal cancer induces an increase in erythrocyte membrane-bound IgG and may cause immune-related anemia.

Authors - Akihito Kitao
mouseIF

eLife

Piezo1 as a force-through-membrane sensor in red blood cells

Authors - George Vaisey
humanIF

NPJ Regen Med

Ferric citrate and apo-transferrin enable erythroblast maturation with β-globin from hemogenic endothelium

Authors - Soo-Been Jeon
mouseIF

Elife

High-altitude hypoxia exposure inhibits erythrophagocytosis by inducing macrophage ferroptosis in the spleen

Authors - Wan-Ping Yang
mouseIHC

Neuron

Single-cell dissection of the human blood-brain barrier and glioma blood-tumor barrier

Authors - Yuan Xie
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