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CoraLite® Plus 488-conjugated Band 3/AE 1 Polyclonal antibody

Band 3/AE 1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28131

Print datasheet

Synonyms

band 3, band 3/ AE1, SLC4A1, Band 3 anion transport protein, Anion exchanger 1

验证数据展示

  • IF Staining of human placenta using CL488-28131

    IF Staining of human placenta using CL488-28131

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 Band 3/AE 1 antibody (CL488-28131) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human placenta using CL488-28131

    IF Staining of human placenta using CL488-28131

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using CoraLite® Plus 488 Band 3/ AE1 antibody (CL488-28131) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive IF-P detected inhuman placenta tissue
Planning an IHC experiment? We recommend our IHCeasy Band 3/AE1 Ready-To-Use IHC Kit. Band 3/AE1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 28131-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-28131 targets Band 3/AE 1 in IF-P applications and shows reactivity with human samples.

Tested Applications IF-P Application Description
Tested Reactivity human
Immunogen Band 3/AE 1 fusion protein Ag27878 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name solute carrier family 4, anion exchanger, member 1 (erythrocyte membrane protein band 3, Diego blood group)
Synonyms band 3, band 3/ AE1, SLC4A1, Band 3 anion transport protein, Anion exchanger 1
Calculated Molecular Weight 911 aa, 102 kDa
Observed Molecular Weight 95-100 kDa
GenBank Accession NumberBC101570
Gene Symbol band 3
Gene ID (NCBI) 6521
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP02730
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Anion exchanger 1 (AE1), also known as band 3, is a 95-100 kDa transmembrane glycoprotein which is abundantly expressed in erythrocyte plasma membrane and mediates the electroneutral exchange of Cl- and HCO3-. It contains a 52-55 kDa C-terminal membrane crossing domain and a 43 kDa N-terminal cytoplasmic domain. Degradation of band 3 protein occurred during erythrocyte aging. The oxidative stress can also activate the proteolysis of band 3 through caspases.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Band 3/AE 1 antibody CL488-28131Download protocol
Standard Protocols
Click here to view our Standard Protocols