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验证数据展示

  • IF Staining of A549 using 82115-2-RR (same clone as 82115-2-PBS)

    IF Staining of A549 using 82115-2-RR (same clone as 82115-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82115-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 82115-2-RR (same clone as 82115-2-PBS)

    IF Staining of A549 using 82115-2-RR (same clone as 82115-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82115-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82115-2-RR (same clone as 82115-2-PBS)

    FC experiment of HeLa using 82115-2-RR (same clone as 82115-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82115-2-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 82115-2-RR (same clone as 82115-2-PBS)

    FC experiment of A549 using 82115-2-RR (same clone as 82115-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82115-2-PBS in a different storage buffer formulation.

SLC7A11/xCT Recombinant antibody, PBS Only

SLC7A11/xCT Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82115-2-PBS

产品说明书

CloneNo. 230227C5

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

82115-2-PBS targets SLC7A11/xCT in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SLC7A11/xCT fusion protein Ag25431 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
别名 SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-
计算分子量 55 kDa
GenBank蛋白编号BC012087
基因名称 SLC7A11
Gene ID (NCBI) 23657
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPY5
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.