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SLC7A11/xCT Recombinant antibody

SLC7A11/xCT Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

230227C5

Cat no : 82115-2-RR

Print datasheet

Synonyms

SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

验证数据展示

  • IF Staining of A549 using 82115-2-RR

    IF Staining of A549 using 82115-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A549 using 82115-2-RR

    IF Staining of A549 using 82115-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 82115-2-RR

    FC experiment of HeLa using 82115-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A549 using 82115-2-RR

    FC experiment of A549 using 82115-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, HeLa cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82115-2-RR targets SLC7A11/xCT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SLC7A11/xCT fusion protein Ag25431 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
Synonyms SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-
Calculated Molecular Weight 55 kDa
GenBank Accession NumberBC012087
Gene Symbol SLC7A11
Gene ID (NCBI) 23657
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UPY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.

实验方案

Product Specific Protocols
IF protocol for SLC7A11/xCT antibody 82115-2-RRDownload protocol
FC protocol for SLC7A11/xCT antibody 82115-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Assist Reprod Genet

PDLIM3 knockdown promotes ferroptosis in endometriosis progression via inducing Gli1 degradation and blocking Hedgehog signaling pathway

Authors - Mingwei Liu
View Article