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验证数据展示

  • IF Staining of A549 using 82115-2-RR

    IF Staining of A549 using 82115-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of A549 using 82115-2-RR

    IF Staining of A549 using 82115-2-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using SLC7A11 antibody (82115-2-RR, Clone: 230227C5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HeLa using 82115-2-RR

    FC experiment of HeLa using 82115-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A549 using 82115-2-RR

    FC experiment of A549 using 82115-2-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Anti-Human SLC7A11 (82115-2-RR, Clone:230227C5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

SLC7A11/xCT Recombinant antibody

SLC7A11/xCT Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 82115-2-RR

产品说明书

CloneNo. 230227C5
引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA and More (1)

别名

SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

82115-2-RR targets SLC7A11/xCT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性human
免疫原 SLC7A11/xCT fusion protein Ag25431 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
别名 SLC7A11, xCT, Cystine/glutamate transporter, CCBR1, Amino acid transport system xc-
计算分子量 55 kDa
GenBank蛋白编号BC012087
基因名称 SLC7A11
Gene ID (NCBI) 23657
RRIDAB_3670516
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9UPY5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC7A11 (also known as xCT) is a membrane transporter involved in the uptake of cystine. It promotes glutathione synthesis through the uptake of cystine and the concomitant release of glutamate. This, in turn, protects cells from oxidative stress, maintains cellular redox balance, and prevents cell death induced by lipid peroxidation. SLC7A11 has been identified as a potential target for cancer therapeutics. It is overexpressed in multiple malignant tumors and is closely associated with the growth, prognosis, metastasis, and treatment response of various cancers including lung cancer.

实验方案

Product Specific Protocols
IF protocol for SLC7A11/xCT antibody 82115-2-RRDownload protocol
FC protocol for SLC7A11/xCT antibody 82115-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Immunol

A disulfidptosis-related lncRNA signature for analyzing tumor microenvironment and clinical prognosis in hepatocellular carcinoma

Authors - Haishui Zheng
View Article