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验证数据展示

  • IF Staining of HepG2 using CL488-19743

    IF Staining of HepG2 using CL488-19743

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SLK antibody (CL488-19743) at dilution of 1:200, CL594-Phalloidin (red).

CoraLite® Plus 488-conjugated SLK Polyclonal antibody

SLK Polyclonal Antibody for IF/ICC
Cat No. CL488-19743

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

bA16H23.1, CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 19743-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-19743 targets SLK in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 STE20-like kinase (yeast)
别名 bA16H23.1, CTCL tumor antigen se20 9, hSLK, KIAA0204, LOSK, se20 9, SLK, STE20 like kinase, STE20 like kinase (yeast), STE20 related kinase, STK2
计算分子量 143 kDa
观测分子量 143-160 kDa, 210-220 kDa
GenBank蛋白编号NM_014720
基因名称 SLK
Gene ID (NCBI) 9748
RRIDAB_3672708
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2G2
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLK, also named as KIAA0204 and STK2, belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. SLK plays an important role in cell proliferation and cytoskeletal remodeling. In fibroblasts, SLK has been shown to respond to FAK/Src signaling and regulate focal adhesion turnover through Paxillin phosphorylation. Full-length SLK (~220 kDa) has also been shown to be essential for embryonic development. In myoblasts, the overexpression of a dominant negative SLK is sufficient to block myoblast fusion. (PMID: 28153048, PMID: 25882817, PMID: 23815977)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SLK antibody CL488-19743Download protocol
Standard Protocols
Click here to view our Standard Protocols