CoraLite®594-conjugated SLP76 Monoclonal antibody

SLP76 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66465

产品说明书

CloneNo. 4H10B1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

LCP2, 4H10B1, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66465-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66465 targets SLP76 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原 SLP76 fusion protein Ag3432 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lymphocyte cytosolic protein 2 (SH2 domain containing leukocyte protein of 76kDa)
别名 LCP2, 4H10B1, SLP 76 tyrosine phosphoprotein, SLP 76, SH2 domain-containing leukocyte protein of 76 kDa
计算分子量 533 aa, 60 kDa
GenBank蛋白编号BC016618
基因名称 SLP76
Gene ID (NCBI) 3937
RRIDAB_2883584
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ13094
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

LCP2, also named as SLP76, is an adapter protein that acts as a substrate of the T cell antigen receptor (TCR)-activated protein tyrosine kinase pathway. It contains an N-terminal leucine Zip motif, a tyrosine-rich domain, a proline-rich domain and a C-terminal SH2 domain. It is constitutively associated with the growth factor receptor-bound protein 2 (Grb2)-related adapter protein 2 (Grap2, a.k.a. GADS) through the interaction of its proline residues with one of the SH3 domains of Grap2. SLP76 associates with growth factor receptor bound protein 2 (Grap2), and is thought to play a role TCR-mediated intracellular signal transduction. SLP76 is also expressed by mast cells, and mast cell activation was markedly impaired in SLP76-deficient mice.

实验方案

Product Specific Protocols
IF protocol for CL594 SLP76 antibody CL594-66465Download protocol
Standard Protocols
Click here to view our Standard Protocols
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