CoraLite® Plus 750-conjugated SMAD1 Monoclonal antibody
SMAD1 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
2D10B8
验证数据展示
经过测试的应用
| Positive WB detected in | HSC-T6 cells |
Planning an IHC experiment? We recommend our IHCeasy SMAD1 Ready-To-Use IHC Kit. SMAD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66559-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-66559 targets SMAD1 in WB applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | human, mouse, rat |
| Immunogen | SMAD1 fusion protein Ag0701 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SMAD family member 1 |
| Synonyms | |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC001878 |
| Gene Symbol | SMAD1 |
| Gene ID (NCBI) | 4086 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q15797 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 SMAD1 antibody CL750-66559 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |