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验证数据展示

  • WB analysis using 83169-5-RR (same clone as 83169-5-PBS)

    WB analysis using 83169-5-RR (same clone as 83169-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83169-5-RR (SMAD4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    IF Staining of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (83169-5-RR, Clone: 240026G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    FC experiment of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SMAD4 Recombinant antibody (83169-5-RR, Clone:240026G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 83169-5-RR (same clone as 83169-5-PBS)

    WB analysis of HEK-293 using 83169-5-RR (same clone as 83169-5-PBS)

    WB result of SMAD4 antibody (83169-5-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD4 transfected HEK-293 cells. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00267-4

    Sandwich ELISA standard curve of MP00267-4

    Sandwich ELISA standard curve of MP00267-4, Human SMAD4 Monoclonal Matched Antibody Pair - PBS only. 83169-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0299. 83169-6-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP00267-4

    Sample test of MP00267-4

    The mean human SMAD4 concentration was determined to be 9,362.0 pg/mL in HepG2 cell extract based on a 6.0 mg/mL extract load and 4,272.2 pg/mL in HL-60 cell extract based on a 3 mg/mL extract load.

  • Sample test of MP00267-4

    Sample test of MP00267-4

    Serum of sixteen individual healthy human donors were measured. The human SMAD4 concentration of detected samples was determined to be 47.3 pg/mL with a range of ND - 148.8 pg/mL.

  • Affinity and Kinetic Characterization of 83169-5-RR

    Affinity and Kinetic Characterization of 83169-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83169-5-RR against Human SMAD4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

SMAD4 Recombinant antibody, PBS Only (Capture)

SMAD4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83169-5-PBS

产品说明书

CloneNo. 240026G9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

别名

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

产品信息

83169-5-PBS targets SMAD4 as part of a matched antibody pair:

MP00267-4: 83169-5-PBS capture and 83169-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse
免疫原 SMAD4 fusion protein Ag0299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD family member 4
别名 MADH4, MAD homolog 4, JIP, hSMAD4, DPC4
计算分子量 60 kDa
观测分子量 63-70 kDa
GenBank蛋白编号BC002379
基因名称 SMAD4
Gene ID (NCBI) 4089
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ13485
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.