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SMAD4 Recombinant antibody, PBS Only

SMAD4 Recombinant Antibody for WB, IF, FC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, FC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240026G9

Cat No : 83169-5-PBS

Print datasheet

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

验证数据展示

  • WB analysis using 83169-5-RR (same clone as 83169-5-PBS)

    WB analysis using 83169-5-RR (same clone as 83169-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83169-5-RR (SMAD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    IF Staining of HepG2 using 83169-5-RR (same clone as 83169-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (83169-5-RR, Clone: 240026G9 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

  • FC experiment of K562 using 83169-5-RR (same clone as 83169-5-PBS)

    FC experiment of K562 using 83169-5-RR (same clone as 83169-5-PBS)

    1x10^6 K562 were intracellularly stained with 0.25 ug SMAD4 Recombinant antibody (83169-5-RR, Clone:240026G9) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83169-5-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

产品信息

The immunogen of 83169-5-PBS is SMAD4 Fusion Protein expressed in E. coli.

Tested Applications WB, IF, FC, Indirect ELISA Application Description
Tested Reactivity Human
Immunogen SMAD4 fusion protein Ag0299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 4
Synonyms DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4
Calculated Molecular Weight 60 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13485
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.