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SMAD4 Recombinant antibody

SMAD4 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240026G9

Cat no : 83169-5-RR

Print datasheet

Synonyms

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

验证数据展示

  • WB analysis using 83169-5-RR

    WB analysis using 83169-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83169-5-RR (SMAD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 83169-5-RR

    IF Staining of HepG2 using 83169-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMAD4 antibody (83169-5-RR, Clone: 240026G9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of HepG2 using 83169-5-RR

    FC experiment of HepG2 using 83169-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug SMAD4 Recombinant antibody (83169-5-RR, Clone:240026G9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83169-5-RR

    Affinity and Kinetic Characterization of 83169-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83169-5-RR against Human SMAD4 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NIH/3T3 cells, A549 cells, PC-3 cells, MCF-7 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83169-5-RR is SMAD4 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity Human, mouse
Immunogen SMAD4 fusion protein Ag0299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 4
Synonyms DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 63-70 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ13485
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.

实验方案

Product Specific Protocols
WB protocol for SMAD4 antibody 83169-5-RRDownload protocol
IF protocol for SMAD4 antibody 83169-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols