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CoraLite® Plus 488-conjugated SMAD4 Recombinant antibody

SMAD4 Recombinant Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240026G9

Cat No : CL488-83169-5

Print datasheet

Synonyms

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

验证数据展示

  • IF Staining of HepG2 using CL488-83169-5

    IF Staining of HepG2 using CL488-83169-5

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SMAD4 antibody (CL488-83169-5, Clone: 240026G9 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-83169-5

    FC experiment of HepG2 using CL488-83169-5

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Smad4 Recombinant Antibody (CL488-83169-5, Clone:240026G9)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83169-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL488-83169-5 is SMAD4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity Human, mouse
Immunogen SMAD4 fusion protein Ag0299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 4
Synonyms MADH4, MAD homolog 4, JIP, hSMAD4, DPC4
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 63-70 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13485
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SMAD4 antibody CL488-83169-5Download protocol
FC protocol for CL Plus 488 SMAD4 antibody CL488-83169-5Download protocol
Standard Protocols
Click here to view our Standard Protocols