验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83169-5-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-83169-5 targets SMAD4 in IF/ICC, FC (Intra) applications and shows reactivity with Human, mouse samples.
| 经测试应用 | IF/ICC, FC (Intra) Application Description |
| 经测试反应性 | Human, mouse |
| 免疫原 | SMAD4 fusion protein Ag0299 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | SMAD family member 4 |
| 别名 | MADH4, MAD homolog 4, JIP, hSMAD4, DPC4 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 63-70 kDa |
| GenBank蛋白编号 | BC002379 |
| 基因名称 | SMAD4 |
| Gene ID (NCBI) | 4089 |
| RRID | AB_3673212 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13485 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF-mediated phosphorylation of other Smad family members.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SMAD4 antibody CL488-83169-5 | Download protocol |
| FC protocol for CL Plus 488 SMAD4 antibody CL488-83169-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |