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SMAD4 Recombinant antibody, PBS Only (Detector)

SMAD4 Uni-rAbTM Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240026A4

Cat no : 83169-6-PBS

Print datasheet

Synonyms

MADH4, MAD homolog 4, JIP, hSMAD4, DPC4

验证数据展示

  • Sandwich ELISA standard curve of MP00267-4

    Sandwich ELISA standard curve of MP00267-4

    Sandwich ELISA standard curve of MP00267-4, Human SMAD4 Monoclonal Matched Antibody Pair - PBS only. 83169-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0299. 83169-6-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

  • Sample test of MP00267-4

    Sample test of MP00267-4

    The mean human SMAD4 concentration was determined to be 9,362.0 pg/mL in HepG2 cell extract based on a 6.0 mg/mL extract load and 4,272.2 pg/mL in HL-60 cell extract based on a 3 mg/mL extract load.

  • Sample test of MP00267-4

    Sample test of MP00267-4

    Serum of sixteen individual healthy human donors were measured. The human SMAD4 concentration of detected samples was determined to be 47.3 pg/mL with a range of ND - 148.8 pg/mL.

Planning an IHC experiment? We recommend our IHCeasy SMAD4 Ready-To-Use IHC Kit. SMAD4 primary antibody included.

产品信息

83169-6-PBS targets SMAD4 as part of a matched antibody pair:

MP00267-4: 83169-5-PBS capture and 83169-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen SMAD4 fusion protein Ag0299 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD family member 4
Synonyms MADH4, MAD homolog 4, JIP, hSMAD4, DPC4
Calculated Molecular Weight 60 kDa
GenBank Accession NumberBC002379
Gene Symbol SMAD4
Gene ID (NCBI) 4089
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13485
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C