验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, HT-29 cells, Caco-2 cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy SMAD5 Ready-To-Use IHC Kit. SMAD5 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| CoIP | See 1 publications below |
产品信息
67052-1-Ig targets SMAD5 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | Human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | SMAD5 fusion protein Ag28699 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | SMAD family member 5 |
| 别名 | DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5 |
| 计算分子量 | 465 aa, 52 kDa |
| 观测分子量 | 52 kDa |
| GenBank蛋白编号 | BC009682 |
| 基因名称 | SMAD5 |
| Gene ID (NCBI) | 4090 |
| RRID | AB_2882365 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99717 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMAD5 antibody 67052-1-Ig | Download protocol |
| IHC protocol for SMAD5 antibody 67052-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Cell Physiol HIF-1α plays an essential role in BMP9-mediated osteoblast differentiation through the induction of a glycolytic enzyme, PDK1. | ||
Mol Cell Endocrinol miR-122-5p targets GREM2 to protect against glucocorticoid-induced endothelial damage through the BMP signaling pathway. | ||
FEBS Lett EGR1 plays an important role in BMP9-mediated osteoblast differentiation by promoting SMAD1/5 phosphorylation. | ||
Appl Bionics Biomech Exosomes Derived from SW480-Resistant Colon Cancer Cells Are Promote Angiogenesis via BMP-2/Smad5 Signaling Pathway. | ||
