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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)

    WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67052-1-Ig (SMAD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD5 antibody included

SMAD5 Monoclonal antibody, PBS Only

SMAD5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67052-1-PBS

产品说明书

CloneNo. 1A6A4

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMAD5 Ready-To-Use IHC Kit. SMAD5 primary antibody included.

产品信息

67052-1-PBS targets SMAD5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 SMAD5 fusion protein Ag28699 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SMAD family member 5
别名 DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5
计算分子量 465 aa, 52 kDa
观测分子量 52 kDa
GenBank蛋白编号BC009682
基因名称 SMAD5
Gene ID (NCBI) 4090
RRIDAB_2882365
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ99717
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.