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SMAD5 Monoclonal antibody, PBS Only

SMAD5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A6A4

Cat no : 67052-1-PBS

Print datasheet

Synonyms

DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5

验证数据展示

  • WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)

    WB analysis using 67052-1-Ig (same clone as 67052-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67052-1-Ig (SMAD5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    IHC staining of human breast cancer using 67052-1-Ig (same clone as 67052-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67052-1-Ig (SMAD5 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67052-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMAD5 antibody included
Planning an IHC experiment? We recommend our IHCeasy SMAD5 Ready-To-Use IHC Kit. SMAD5 primary antibody included.

产品信息

67052-1-PBS targets SMAD5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen SMAD5 fusion protein Ag28699 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SMAD family member 5
Synonyms DKFZp781C1895, DKFZp781O1323, Dwfc, hSmad5, JV5 1, MAD homolog 5, MADH5, Mothers against DPP homolog 5, SMAD 5, SMAD family member 5, SMAD5
Calculated Molecular Weight 465 aa, 52 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC009682
Gene Symbol SMAD5
Gene ID (NCBI) 4090
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ99717
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD5, also named as MADH5 and JV5-1, is a transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). It plays a critical role in the signaling pathway by which TGF-beta inhibits the proliferation of human hematopoietic progenitor cells.