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验证数据展示

  • WB analysis using 68594-1-Ig

    WB analysis using 68594-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68594-1-Ig (SMAD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HeLa using 68594-1-Ig

    IF Staining of HeLa using 68594-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMAD7 antibody (68594-1-Ig, Clone: 3D1C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • FC experiment of U2OS using 68594-1-Ig

    FC experiment of U2OS using 68594-1-Ig

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMAD7 Monoclonal antibody (68594-1-Ig, Clone:3D1C3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SMAD7 Monoclonal antibody

SMAD7 Monoclonal Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 68594-1-Ig

产品说明书

CloneNo. 3D1C3

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, FC (Intra), ELISA

别名

MADH7, MAD homolog 8, MAD homolog 7, hSMAD7, CRCS3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig kidney tissue, mouse brain tissue, rat brain tissue, rabbit brain tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SMAD7 Ready-To-Use IHC Kit. SMAD7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68594-1-Ig targets SMAD7 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 SMAD7 fusion protein Ag13688 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 SMAD family member 7
别名 MADH7, MAD homolog 8, MAD homolog 7, hSMAD7, CRCS3
计算分子量 426 aa, 46 kDa
观测分子量45-55 kDa
GenBank蛋白编号BC074819
基因名称 SMAD7
Gene ID (NCBI) 4092
RRIDAB_3085293
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15105
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

实验方案

Product Specific Protocols
WB protocol for SMAD7 antibody 68594-1-IgDownload protocol
IF protocol for SMAD7 antibody 68594-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols