• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SMAD7 Monoclonal antibody, PBS Only

SMAD7 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat, rabbit, pig

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3D1C3

Cat no : 68594-1-PBS

Print datasheet

Synonyms

CRCS3, FLJ16482, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7, SMAD7

验证数据展示

  • WB analysis using 68594-1-Ig (same clone as 68594-1-PBS)

    WB analysis using 68594-1-Ig (same clone as 68594-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68594-1-Ig (SMAD7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68594-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMAD7 Ready-To-Use IHC Kit. SMAD7 primary antibody included.

产品信息

68594-1-PBS targets SMAD7 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit, pig
Immunogen SMAD7 fusion protein Ag13688 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name SMAD family member 7
Synonyms CRCS3, FLJ16482, hSMAD7, MAD homolog 7, MAD homolog 8, MADH7, MADH8, Mothers against DPP homolog 7, Mothers against DPP homolog 8, SMAD 7, SMAD family member 7, SMAD7
Calculated Molecular Weight 426 aa, 46 kDa
Observed Molecular Weight45-55 kDa
GenBank Accession NumberBC074819
Gene Symbol SMAD7
Gene ID (NCBI) 4092
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO15105
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMAD7, also named as Mothers against decapentaplegic homolog 7, is a 426 amino acid protein, which belongs to the dwarfin/SMAD family. SMAD7 Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). SMAD7 as antagonist of signaling by TGF-beta type 1 receptor superfamily members has been shown to inhibit TGF-beta and activin signaling by associating with their receptors thus preventing SMAD2 access. SMAD7 functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex and also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. SMAD7 positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.