验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, MDA-MB-453s cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SMARCA2 Ready-To-Use IHC Kit. SMARCA2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| CoIP | See 1 publications below |
产品信息
26613-1-AP targets SMARCA2-Specific in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | SMARCA2-Specific fusion protein Ag25157 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2 |
| 别名 | SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B |
| 计算分子量 | 181 kDa |
| 观测分子量 | 180-190 kDa |
| GenBank蛋白编号 | NM_003070 |
| 基因名称 | SMARCA2 |
| Gene ID (NCBI) | 6595 |
| RRID | AB_2880575 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P51531 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SMARCA2-Specific antibody 26613-1-AP | Download protocol |
| IHC protocol for SMARCA2-Specific antibody 26613-1-AP | Download protocol |
| IF protocol for SMARCA2-Specific antibody 26613-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Nucleoporin Seh1 Interacts with Olig2/Brd7 to Promote Oligodendrocyte Differentiation and Myelination. | ||
Stem Cells Chromatin Remodeling Factor BRG1 Regulates Stemness and Chemosensitivity of Glioma Initiating Cells. | ||
Biochem Biophys Res Commun Chromatin remodeler BRM is a key mediator of leucine-stimulated mTOR gene transcription in mouse mammary epithelial cells | ||
Nat Commun Prion-like domain mediated phase separation of ARID1A promotes oncogenic potential of Ewing's sarcoma | ||
Cell Rep SWI/SNF complex-mediated ZNF410 cooperative binding maintains chromatin accessibility and enhancer activity |
