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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SMARCA2-Specific Polyclonal antibody

SMARCA2-Specific Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-26613

Print datasheet

Synonyms

SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B

验证数据展示

  • IF Staining of HepG2 using CL488-26613

    IF Staining of HepG2 using CL488-26613

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 SMARCA2-Specific antibody (CL488-26613) at dilution of 1:400, CL594-Phalloidin (red).

  • FC experiment of HeLa using CL488-26613

    FC experiment of HeLa using CL488-26613

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SMARCA2-Specific (CL488-26613) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SMARCA2 Ready-To-Use IHC Kit. SMARCA2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 26613-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-26613 targets SMARCA2-Specific in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen SMARCA2-Specific fusion protein Ag25157 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Synonyms SMARCA2, EC:3.6.4.-, BRM, BRG1-associated factor 190B, BAF190B
Calculated Molecular Weight 181 kDa
Observed Molecular Weight 180-190 kDa
GenBank Accession NumberNM_003070
Gene Symbol SMARCA2
Gene ID (NCBI) 6595
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51531
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SMARCA2-Specific antibody CL488-26613Download protocol
Standard Protocols
Click here to view our Standard Protocols