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CoraLite® Plus 488-conjugated SMARCA4 Monoclonal antibody
SMARCA4 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human
Applications
IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2E6B6
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SMARCA4 Ready-To-Use IHC Kit. SMARCA4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66561-1-Ig
推荐稀释比
Application | Dilution |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66561 targets SMARCA4 in IF/ICC applications and shows reactivity with Human samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | Human |
Immunogen | SMARCA4 fusion protein Ag16256 种属同源性预测 |
Host / Isotype | Mouse / IgG2b |
Class | Monoclonal |
Type | Antibody |
Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 |
Synonyms | ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, FLJ39786, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1 |
Calculated Molecular Weight | 1647 aa, 185 kDa |
GenBank Accession Number | BC150298 |
Gene Symbol | SMARCA4 |
Gene ID (NCBI) | 6597 |
RRID | AB_2883355 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P51532 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL488(Ex/Em 488 nm/515 nm) conjugated.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 SMARCA4 antibody CL488-66561 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |