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验证数据展示

  • WB analysis of HeLa using 20650-1-AP (same clone as 20650-1-PBS)

    WB analysis of HeLa using 20650-1-AP (same clone as 20650-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20650-1-AP (SMARCA4/BRG1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 20650-1-AP (same clone as 20650-1-PBS)

    WB analysis of HepG2 using 20650-1-AP (same clone as 20650-1-PBS)

    WB result of SMARCA4/BRG1 antibody (20650-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMARCA4/BRG1 transfected HepG2 cells. This data was developed using the same antibody clone with 20650-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

SMARCA4/BRG1 Polyclonal antibody, PBS Only

SMARCA4/BRG1 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 20650-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMARCA4 Ready-To-Use IHC Kit. SMARCA4 primary antibody included.

产品信息

20650-1-PBS targets SMARCA4/BRG1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
别名 SMARCA4, BRG1 associated factor 190A, BRG1, BAF190A, BAF190
计算分子量 185 kDa
观测分子量 180 kDa
GenBank蛋白编号NM_001128844
基因名称 SMARCA4
Gene ID (NCBI) 6597
RRIDAB_10694827
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP51532
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. The antibody is specific to SMARCA4.