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验证数据展示

  • WB analysis using 67979-1-Ig

    WB analysis using 67979-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67979-1-Ig (SMARCB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of mouse stomach using 67979-1-Ig

    IHC staining of mouse stomach using 67979-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67979-1-Ig (SMARCB1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse stomach using 67979-1-Ig

    IHC staining of mouse stomach using 67979-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 67979-1-Ig (SMARCB1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

SMARCB1 Monoclonal antibody

SMARCB1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67979-1-Ig
CloneNo. 1D10A4

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

INI1, hSNFS, hSNF5, BRG1-associated factor 47, BAF47

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inNCCIT cells, LNcaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67979-1-Ig targets SMARCB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SMARCB1 fusion protein Ag28911 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1
Synonyms INI1, hSNFS, hSNF5, BRG1-associated factor 47, BAF47
Calculated Molecular Weight 44 kDa
Observed Molecular Weight40-45 kDa
GenBank Accession NumberNM_003073
Gene Symbol SMARCB1
Gene ID (NCBI) 6598
RRIDAB_2918728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ12824
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCB1 is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.

实验方案

Product Specific Protocols
WB protocol for SMARCB1 antibody 67979-1-IgDownload protocol
IHC protocol for SMARCB1 antibody 67979-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols