验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 67979-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67979 targets SMARCB1 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | FC (Intra) Application Description |
| Tested Reactivity | Human, mouse, rat |
| Immunogen | SMARCB1 fusion protein Ag28911 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily b, member 1 |
| Synonyms | SMARCB1, SNF5, SNF5L1, Snr1, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1, 1D10A4, BAF47, BRG1-associated factor 47, hSNF5, hSNFS, INI1, INI-1, RDT, Sfh1p |
| Calculated Molecular Weight | 44 kDa |
| Observed Molecular Weight | 40-45 kDa |
| GenBank Accession Number | NM_003073 |
| Gene Symbol | SMARCB1 |
| Gene ID (NCBI) | 6598 |
| RRID | AB_2923846 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q12824 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SMARCB1 is part of a complex that relieves repressive chromatin structures, allowing the transcriptional machinery to access its targets more effectively. The encoded nuclear protein may also bind to and enhance the DNA joining activity of HIV-1 integrase. This gene has been found to be a tumor suppressor and mutations in it have been associated with malignant rhabdoid tumors. Two transcript variants encoding different isoforms have been found for this gene.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 SMARCB1 antibody CL488-67979 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |