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验证数据展示

  • WB analysis using CL488-66116

    WB analysis using CL488-66116

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66116 (SMARCD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated SMARCD1 Monoclonal antibody

SMARCD1 Monoclonal Antibody for WB
Cat No. CL488-66116

产品说明书

CloneNo. 2F2G6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB

别名

BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66116-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66116 targets SMARCD1 in WB applications and shows reactivity with human samples.

经测试应用 WB Application Description
经测试反应性 human
免疫原 SMARCD1 fusion protein Ag19100 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1
别名 BAF60A, BRG1 associated factor 60A, CRACD1, Rsc6p, SMARCD1, SWI/SNF complex 60 kDa subunit
计算分子量 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC009368
基因名称 SMARCD1
Gene ID (NCBI) 6602
RRIDAB_3084208
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96GM5
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMARCD1 (SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 1), also known as BAF60a (BRG1-associated factor 60A),Rsc6p or CRACD1, is a 476 amino acid protein that localizes to the nucleus and contains one SWIB domain. Expressed ubiquitously with notable expression in liver, brain, muscle, lung, kidney, pancreas and placenta, SMARCD1 functions as a component of the BAF complex and, in conjunction with a variety of other proteins, plays an essential role in chromatin remodeling. In addition, SMARCD1 influences vitamin D-mediated transcriptional activity and is thought to provide a link between the vitamin D receptor (VDR) and SWI/SNF chromatin remodeling complexes. Multiple isoforms of BAF60a exist due to alternative splicing events.

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 SMARCD1 antibody CL488-66116Download protocol
Standard Protocols
Click here to view our Standard Protocols