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SMARCE1 Monoclonal antibody, PBS Only

SMARCE1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1F3D6

Cat No : 66182-1-PBS

Print datasheet

Synonyms

BAF57, BRG1 associated factor 57, SMARCE1

验证数据展示

  • WB analysis of Jurkat using 66182-1-Ig (same clone as 66182-1-PBS)

    WB analysis of Jurkat using 66182-1-Ig (same clone as 66182-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

    IHC staining of human breast cancer using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66182-1-Ig (SMARCE1 Antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMARCE1 antibody included
  • IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

    IF Staining of HEK-293 using 66182-1-Ig (same clone as 66182-1-PBS)

    Immunofluorescent analysis of HEK-293 cells using 66182-1-Ig (SMARCE1 antibody) at dilution of 1:50 and FITC-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66182-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SMARCE1 Ready-To-Use IHC Kit. SMARCE1 primary antibody included.

产品信息

66182-1-PBS targets SMARCE1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse, rat
Immunogen SMARCE1 fusion protein Ag1161 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
Synonyms BAF57, BRG1 associated factor 57, SMARCE1
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC007082
Gene Symbol SMARCE1
Gene ID (NCBI) 6605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ969G3
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.