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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 66987-1-Ig (same clone as 66987-1-PBS)

    WB analysis using 66987-1-Ig (same clone as 66987-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66987-1-Ig (SMC1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

    IHC staining of human colon cancer using 66987-1-Ig (same clone as 66987-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66987-1-Ig (SMC1A antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with SMC1A antibody included
  • FC experiment of HeLa using 66987-1-Ig (same clone as 66987-1-PBS)

    FC experiment of HeLa using 66987-1-Ig (same clone as 66987-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SMC1A Monoclonal antibody (66987-1-Ig, Clone:1A5D2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66987-1-PBS in a different storage buffer formulation.

SMC1A Monoclonal antibody, PBS Only

SMC1A Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 66987-1-PBS

产品说明书

CloneNo. 1A5D2

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, FC (Intra), ELISA

别名

SMC 1A, SMC 1 alpha, Sb1.8, KIAA0178, DXS423E

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMC1A Ready-To-Use IHC Kit. SMC1A primary antibody included.

产品信息

66987-1-PBS targets SMC1A in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMC1A fusion protein Ag17257 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 structural maintenance of chromosomes 1A
别名 SMC 1A, SMC 1 alpha, Sb1.8, KIAA0178, DXS423E
计算分子量 1233 aa, 143 kDa
观测分子量 150 kDa
GenBank蛋白编号BC112127
基因名称 SMC1A
Gene ID (NCBI) 8243
RRIDAB_2882304
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14683
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.