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验证数据展示

  • IF Staining of human colon cancer using CL594-66987

    IF Staining of human colon cancer using CL594-66987

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 SMC1A antibody (CL594-66987, Clone: 1A5D2 ) at dilution of 1:200.

CoraLite®594-conjugated SMC1A Monoclonal antibody

SMC1A Monoclonal Antibody for IF-P
Cat No. CL594-66987

产品说明书

CloneNo. 1A5D2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

IF-P

别名

CDLS2, DKFZp686L19178, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy SMC1A Ready-To-Use IHC Kit. SMC1A primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66987-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66987 targets SMC1A in IF-P applications and shows reactivity with Human, mouse, rat samples.

经测试应用 IF-P Application Description
经测试反应性 Human, mouse, rat
免疫原 SMC1A fusion protein Ag17257 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 structural maintenance of chromosomes 1A
别名 CDLS2, DKFZp686L19178, DXS423E, KIAA0178, SB1.8, SMC 1 alpha, SMC 1A, SMC protein 1A, SMC1, SMC1A, SMC1alpha, SMC1L1, SMCB
计算分子量 1233 aa, 143 kDa
观测分子量 150 kDa
GenBank蛋白编号BC112127
基因名称 SMC1A
Gene ID (NCBI) 8243
RRIDAB_2920067
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ14683
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Structural maintenance of chromosomes protein 1A (SMC1A) is involved in chromosome cohesion during cell cycle and in DNA repair. It is a central component of cohesin complex which is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint.

实验方案

Product Specific Protocols
IF protocol for CL594 SMC1A antibody CL594-66987Download protocol
Standard Protocols
Click here to view our Standard Protocols