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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14465-1-AP (same clone as 14465-1-PBS)

    WB analysis using 14465-1-AP (same clone as 14465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IHC staining of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14465-1-AP (SMC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IP experiment of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14465-1-AP (same clone as 14465-1-PBS)

    IP experiment of HeLa using 14465-1-AP (same clone as 14465-1-PBS)

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:4000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

SMC6 Polyclonal antibody, PBS Only

SMC6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14465-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

14465-1-PBS targets SMC6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SMC6 fusion protein Ag5812 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 structural maintenance of chromosomes 6
别名
计算分子量 126 kDa
观测分子量 130 kDa
GenBank蛋白编号BC039828
基因名称 SMC6
Gene ID (NCBI) 79677
RRIDAB_2192792
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96SB8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).