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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 14465-1-AP (same clone as 14465-1-PBS)

    WB analysis using 14465-1-AP (same clone as 14465-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14465-1-AP (SMC6 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IHC staining of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14465-1-AP (SMC6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IP experiment of mouse testis using 14465-1-AP (same clone as 14465-1-PBS)

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 14465-1-AP (same clone as 14465-1-PBS)

    IP experiment of HeLa using 14465-1-AP (same clone as 14465-1-PBS)

    IP result of anti-SMC6 (IP:14465-1-AP, 4ug; Detection:14465-1-AP 1:4000) with HeLa cells lysate 1320 ug. This data was developed using the same antibody clone with 14465-1-PBS in a different storage buffer formulation.

SMC6 Polyclonal antibody, PBS Only

SMC6 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 14465-1-PBS

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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产品信息

14465-1-PBS targets SMC6 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen SMC6 fusion protein Ag5812 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name structural maintenance of chromosomes 6
Synonyms
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039828
Gene Symbol SMC6
Gene ID (NCBI) 79677
RRIDAB_2192792
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SB8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).