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SMC6 Recombinant antibody

SMC6 Uni-rAbTM Recombinant Antibody for WB, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240015F4

Cat no : 83187-3-RR

Print datasheet

Synonyms

SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6

验证数据展示

  • WB analysis using 83187-3-RR

    WB analysis using 83187-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 83187-3-RR

    WB analysis of human testis using 83187-3-RR

    human testis tissue were subjected to SDS PAGE followed by western blot with 83187-3-RR (SMC6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 83187-3-RR

    IF Staining of mouse testis using 83187-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of A431 using 83187-3-RR

    FC experiment of A431 using 83187-3-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SMC6 Recombinant antibody (83187-3-RR, Clone:240015F4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of mouse testis using 83187-3-RR

    IF Staining of mouse testis using 83187-3-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using SMC6 antibody (83187-3-RR, Clone: 240015F4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83187-3-RR

    Affinity and Kinetic Characterization of 83187-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83187-3-RR against Human SMC6 were performed. The affinity constant is 0.428 nM.

经过测试的应用

Positive WB detected inHeLa cells, human testis tissue, NIH/3T3 cells, HEK-293 cells
Positive IF-P detected inmouse testis tissue
Positive FC (Intra) detected inA431 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83187-3-RR targets SMC6 in WB, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF-P, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse
Immunogen SMC6 fusion protein Ag6898 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name structural maintenance of chromosomes 6
Synonyms SMC6L1, SMC-6, SMC protein 6, SMC 6, hSMC6
Calculated Molecular Weight 126 kDa
Observed Molecular Weight 130 kDa
GenBank Accession NumberBC039828
Gene Symbol SMC6
Gene ID (NCBI) 79677
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ96SB8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMC6 (Structural maintenance of chromosomes protein 6) is core component of the SMC5-SMC6 complex that is involved in DNA double-strand breaks by homologous recombination. The complex may promote sister chromatid homologous recombination by recruiting the SMC1-SMC3 cohesin complex to double-strand breaks. The complex is required for telomere maintenance via recombination in ALT (alternative lengthening of telomeres) cell lines and mediates sumoylation of shelterin complex (telosome) components which is proposed to lead to shelterin complex disassembly in ALT-associated PML bodies (APBs) (PMID: 26983541).

实验方案

Product Specific Protocols
WB protocol for SMC6 antibody 83187-3-RRDownload protocol
IF protocol for SMC6 antibody 83187-3-RRDownload protocol
FC protocol for SMC6 antibody 83187-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols